REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
IRDye 680RD Goat anti-Rabbit IgG (H + L)	LI-COR	Cat926-68071
IRDye 800CW Goat anti-Mouse IgG (H + L)	LI-COR	Cat# 926-32210
IRDye 800RD Goat anti-Rat IgG (H + L)	LI-COR	Cat# 925-32219
HCoV-229E Nucleocapsid Antibody	SinoBiological	Cat# 40640-T62
Anti-Coronavirus NL63	Ingenasa	M.30.HCo.I2D4
Anti-Coronavirus Antibody, OC-43 strain	Millipore	Cat# MAB9012
Purified anti-GAPDH Antibody	Biolegend	Cat# 607902
Rabbit polyclonal anti-IFITM1	Cell signaling	Cat# 13126 S
Rabbit polyclonal anti-IFITM2	Cell signaling	Cat# 13530S
Rabbit polyclonal anti-IFITM3	Cell signaling	Cat# 59212S
V5-Tag (E9H8O) Mouse mAb	Cell signaling	Cat# 80076
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody	SinoBiological	Cat# 40588-MM124
Rabbit polyclonal anti-ACE2	Abcam	Cat# ab15348
Mouse monoclonal anti-flag	Sigma-Aldrich	Cat# F1804
Fixable Viability Dye eFluor 780	Thermo Fisher	Cat# 65-0865-14
Rabbit monoclonal Alexa Fluor® 647 anti-CD46	Abcam	Cat# 275208
Rat Monoclonal PerCP-Cy5.5 anti-CD4	Biolegend	Cat# 317428
Bacterial and virus strains
VSVΔG(GFP)∗VSV-G	Klaus Conzelmann	N/A
hCoV-229E	ATCC	Cat# VR-740TM
hCoV-NL63	Lia van der Hoek	N/A
hCoV-OC43	ATCC	Cat# CR-1558TM
Renilla hCoV-229E	Volker Thiel	N/A
BetaCoV/Netherlands/01/NL/2020	European Virus Archive	Cat# 010V 03903
VSVΔG(GFP)∗VSV-G	Klaus Conzelmann	N/A
BetaCoV/Netherlands/01/NL/2020	European Virus Archive	Cat# 010V 03903
BetaCoV/B.1.617.2/Delta	Florian Schmidt (University of Bonn)	N/A
BetaCoV/Munich/BavPat1/2020	GISAID; Wölfel et al., 202047	EPI_ISL_406862
SARS-CoV (rSCV, Frankfurt-1 virus isolate)	Pfefferle et al., 200929	Genbank accession no. AY310120
rMERS-CoV, MERS-CoV/EMC2012	Muth et al., 201748	Genbank accession no. NC_019843.3
Chemicals, peptides, and recombinant proteins
L-Glutamine	Pan Biotech	Cat# P04-80100
DAPI	Sigma-Aldrich	Cat# D9542-1MG; CAS: 28718-90-3
Dulbecco’s Modified Eagle Medium (DMEM)	Gibco	Cat# 41965039
Dulbecco’s Phosphate Buffered Saline (PBS)	Thermo Fisher	Cat# 14190094
Minimum Essential Medium Eagle (MEM)	Sigma-Aldrich	Cat# M4655
Opti-MEM	Gibco	Cat# M3942
Roswell Park Memorial Institute (RPMI)	Gibco	Cat# 22400089
Penicillin-Streptomycin	Thermo Fisher	Cat# 15140122
non-essential amino acids	Sigma-Aldrich	Cat# M7145
Sodium pyruvate	Sigma-Aldrich	Cat# S8636
FCS	Gibco	Cat# 10270106
Trypsin EDTA	Gibco	Cat# T3924
Versene	Thermo Fisher	Cat# 15040066
TMB peroxidase substrate (Sure blue)	Medac	Cat# 52-00-04
BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit	BD Biosciences	Cat# 554714
Lipofectamine RNAiMAX	ThermoFisher	Cat# 13778075
SuperScript™ III Platinum™ One-Step qRT-PCR Kit	Invitrogen™	Cat# 11732088
Renilla Luciferase Assay kit	Promega	Cat# E2810
Quick RNA Miniprep Kit	ZYMO research	Cat# R1055
QIAamp Viral RNA Kits	QIAGEN	Cat# 52906
Luciferase Assay Kit	Promega	Cat# E1500
Mowiol 4-88	ROTH	Cat# 0713.1
Blocker Casein in PBS	Thermo Fisher	Cat# 37528
TaqMan Fast Virus 1-Step Master Mix	ThermoFisher	Cat# 4444436
Tween 20	Sigma-Aldrich	Cat# P7949500ML
Triton X-100	Sigma-Aldrich	Cat#T9284 100ML
GAPD (GAPDH) Endogenous Control	ThermoFisher	Cat# 4352338E
β-Mercaptoethanol	Sigma-Aldrich	Cat# 444203
Immobilon-FL PVDF membranes	Merck Millipore	Cat# IPVH00010
Paraformaldehyde	Merck, Darmstadt	Cat# 104005
TransIT-LT1	Mirus	Cat# MIR2300
NuPAGE 4±12% Bis-Tris Gels	ThermoFisher	Cat# NP0321BOX
Synthetic SARS-CoV-2-RNA	Twist Bioscience	Cat# 102019
RNAiMAX	ThermoFisher	Cat# LMRNA015
Recombinant IFN-α2	pbl assay science	Cat# 11101-1
Recombinant IFN-β	R&D systems	Cat# 8499-IF-010
Recombinant IFN-γ	Sigma-Aldrich	Cat# I3265
Recombinant IFN- λ3	CHO	Cat# 5259-IL-025
Cytochalasin D	ThermoFisher	Cat# PHZ1063
Agarose	Sigma-Aldrich	Cat# A9539
Experimental models: Cell lines
Human: Calu-3	ATCC or M. Frick	Cat# HTB-55 or N/A
Human: Caco2	Holger Barth
Human: Huh7.5	Ralf Bartenschlager
Human: Huh7	Anna-Laura Kretz
LLCMK2	by Lia van der Hoek
HCT8	ATCC	CCL-244TM
Human: HEK293Ts	ATCC	Cat# CRL-3216
Human: HeLa	ATCC	Cat# CRM-CCL-2
African Green Monkey: Vero B4	ATCC	Cat# DSMZ-AC33
African Green Monkey: Vero E6	ATCC	Cat# DSMZ-AC33
Human: Hela-ACE2	This paper	Cat# CRL-1586
Human: A549	Hoffmann et al., 202037
Human: A549-ACE2	Hoffmann et al., 202037
Human: A549-TMPRSS2	Hoffmann et al., 202037
Human: A549-TMPRSS2-ACE2	Hoffmann et al., 202037
Human: TZM-bl	NIH	Cat# ARP-8129
Oligonucleotides
229E Forward: TACTCGAGGTTCCGTCTC GT	Biomers	N/A
229E Reverse: CAAGTGTCACACGGTTGCAG	Biomers	N/A
229E probe: AGC AAA GTT CTG TCT TTG TGG AAA CCA	Biomers	N/A
NL63 Forward: AAACCTCGTTGGAAGCGTGT	Biomers	N/A
NL63 Reverse: CTGTGGAAAACCTTTGGCATC	Biomers	N/A
NL63 Probe: ATGTTATTCAGTGCTTTGGTCCTCGTGAT	Biomers	N/A
OC43 Forward: AGCAACCAGGCTGATGTC AATACC	Biomers	N/A
OC43 Reverse: AGCAGACCTTCCTGAGCCTTCAAT	Biomers	N/A
OC43 Probe: TGACATTGTCGATCGGGACCCAAGTA	Biomers	N/A
HKU-NF forward: TAATCAGACAAGGAACTGATTA	Biomers	N/A
HKU-N reverse: CGAAGGTGTGACTTCCAT G	Biomers	N/A
HKU-N Probe: FAM-GCAAATTGTGCAATTTGCGG-TAMRA	Biomers	N/A
SARS-CoV-1 forward (EMC-upE-F): GCA ACG CGC GAT TCA GTT	Biomers	N/A
SARS-CoV-1 reverse (EMC-upE-R): GCC TCT ACA CGG GAC CCA TA	Biomers	N/A
SARS-CoV-1 probe (EMC-upE-P): FAM-CTC TTCACATAATCGCCCCGAGCTCG-TAMRA	Biomers	N/A
SARS-CoV-2 forward (HKU-NF): TAA TCA GAC AAG GAA CTG ATT A	Biomers	N/A
SARS-CoV-2 reverse (HKU-NR): CGA AGG TGT GAC TTC CAT G	Biomers	N/A
SARS-CoV-2 probe (HKU-NP): FAM-GCA AAT TGT GCA ATT TGC GG-TAMRA	Biomers	N/A
MERS-CoV forward (E-Sarbeco-F): ACA GGT ACG TTA ATA GTT AAT AGC GT	Biomers	N/A
MERS-CoV reverse (E-Sarbeco-R): ATA TTG CAG CAG TAC GCA CAC A	Biomers	N/A
MERS-CoV probe (E-Sarbeco-P): FAM-ACA CTA GCC ATC CTT ACT GCG CTT CG-BBQ	Biomers	N/A
Human ACTB (Beta Actin) Endogenous Control	Applied Biosystems	Cat# 4310881E
CTRL siRNA-01: UAAGGCUAUGAAGAGAUAC	This paper	D-001206-14-20
CTRL siRNA-02: AUGUAUUGGCCUGUAUUAG	This paper	D-001206-14-21
CTRL siRNA-03: AUGAACGUGAAUUGCUCAA	This paper	D-001206-14-22
CTRL siRNA-04: UGGUUUACAUGUCGACUAA	This paper	D-001206-14-23
siRNA IFITM1-01: GUGACAGUCUACCAUAUUA	Dharmacon	D-019543-01
siRNA IFITM1-02: CUUCCAAGGUCCACCGUGA	Dharmacon	D-019543-02
siRNA IFITM1-03: CCUCAUGACCAUUGGAUUC	Dharmacon	D-019543-03
siRNA IFITM1-04: GUUACAGAUAAUACAGGAA	Dharmacon	D-019543-04
siRNA IFITM2-01: CAAACCUUCUCUCCUGUCA	Dharmacon	D-020103-01
siRNA IFITM2-02: UCAAGGAGGAGCAGGAAGU	Dharmacon	D-020103-02
siRNA IFITM2-03: UCGUCCAGGCCCAGCGAUA	Dharmacon	D-020103-03
siRNA IFITM2-04: UGGUCUGGUCCCUGUUCAA	Dharmacon	D-020103-18
siRNA IFITM3-01: ACGUGUUUCUGGUGCUAAA	Dharmacon	D-014116-13
siRNA IFITM3-02: AUGGAUAGAUCAGGAGGCA	Dharmacon	D-014116-14
siRNA IFITM3-03: UGCUGAUCUUCCAGGCCUA	Dharmacon	D-014116-15
siRNA IFITM3-04: UCGUCAUCCCAGUGCUGAU	Dharmacon	D-014116-16
siRNA ACE2-01: GAACAUACCUUUGAAGAGA	Dharmacon	D-005755-01
siRNA ACE2-02: GCACAAAGGUGACAAUGGA	Dharmacon	D-005755-02
siRNA ACE2-03: GAAGGUGGAUGGUCUUUAA	Dharmacon	D-005755-03
siRNA ACE2-04: GAGGGAGACUAUGAAGUA	Dharmacon	D-005755-04
Recombinant DNA
pCG_hCoV-229E-spike C-V5-tag_BFP	Lawrenz et al., 202249
pCG_hCoV-NL63-spike C-V5-tag_BFP	Lawrenz et al., 202249
pCMV3-hCoV-OC43-spike-Flag	Sinobiological	VG40607-CF
pCMV3-hCoV-HKU1-spike	Sinobiological	VG40021-UT
pCG_MERS-CoV S (humanized) C-V5 IRES GFP	Michael Schindler
pCG_SARS-CoV-1 S (hum) C-V5 IRES GFP	Michael Schindler
pCG_SARS-CoV-2-Spike-V5_IRES_eGFP (humanized)	Prelli Bozzo et al., 202122
pCG_IFITM1	Prelli Bozzo et al., 202122	N/A
pCG_IFITM2	Prelli Bozzo et al., 202122	N/A
pCG_IFITM3	Prelli Bozzo et al., 202122	N/A
Mouse Ifitm1(NM_026820) ORF Clone	Genescript	OMU15399D
Mouse Ifitm2(NM_030694) ORF Clone	Genescript	OMU08371D
Mouse Ifitm3(NM_025378) ORF Clone	Genescript	OMU18001D
pCG_IFITM1-IRES_eGFP	Prelli Bozzo et al., 202122	N/A
pCG_IFITM2-IRES_eGFP	Prelli Bozzo et al., 202122	N/A
pCG_IFITM3-IRES_BFP	Prelli Bozzo et al., 202122	N/A
pTwist_EF1a_3Xflag_IFITM1	This paper	N/A
pTwist_EF1a_3Xflag_IFITM2	This paper	N/A
pTwist_EF1a_3Xflag_IFITM3	This paper	N/A
pCDNA3.1_hACE2	This paper	N/A
pCDNA3.1_hCD4	This paper	N/A
pCG_Nef NL4-3_IRES GFP	This paper	N/A
pCG_SARS-CoV2-Spike-IRES_eGFP	Stefan Pöhlmann37	N/A
Software and algorithms
Corel DRAW 2017	Corel Corporation	https://www.coreldraw.com/
GraphPad Prism Version 9	GraphPad Software, Inc.	https://www.graphpad.com
LI-COR Image Studio Lite Version 5.0	LI-COR	www.licor.com/
Fiji (ImageJ) version 1.8	Java	www.imagej.nih.gov/ij