FIG. 2.
Structures of Tn4656 and its deletion derivatives. Abbreviations for restriction sites: B, BamHI; E, EcoRI; H, HindIII; K, KpnI, S, StuI; and Sm, SmaI. (A) Tn4656 and its large deletion derivatives. The location of the xyl genes is based on information from reference 13. A horizontal arrow indicates the direction of transcription of a gene or operon, and the vertical arrows indicate the outermost BamHI sites and the rightmost HindIII and KpnI sites. The res site located upstream of the tnpR gene is not shown for the sake of simplicity. The deleted fragment represented by a thin line was replaced by the pRME1-derived Kmr gene. Tn4656-2926 is loaded on pMT252, and the remaining three Tn4656 derivatives are loaded on pMT258. For details concerning construction of the plasmids, see Table 1 and Materials and Methods. The transposition frequency is expressed as the number of Kmr transconjugants per Tpr transconjugant. (B) Construction of the Tn4656-2890 derivatives. Abbreviations: IRL and IRR, left and right IRs, respectively. The derivatives are not drawn to scale. Construction of pMT2923 from pMT2890 via pMT2916 as an intermediate is described in Materials and Methods. The open and shaded boxes represent the pRME1-derived fragment and the fragment deleted in the descendant transposon, respectively. Note that only the relevant restriction sites derived from pRME1 are shown for the sake of simplicity. The restriction sites in parentheses are not digested by SmaI or StuI.