KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Calreticulin | ThermoFisher | Cat# PA3-900; RRID: AB_325990 |
| c-Myc (9E10) | Santa Cruz | Cat# SC-40; RRID: AB_627268 |
| FLAG (M2) | Sigma | Cat# F-1804; RRID: AB_262044 |
| GFP | Santa Cruz | Cat# SC-9996; RRID: AB_627695 |
| GFP | Developmental Studies Hybridoma Bank | Cat# DSHB-GFP-4C9; RRID: AB_2617422 |
| GFP | ThermoFisher | Cat# A31852; RRID: AB_162553 |
| GM130 | Abcam | Cat# Ab169276; RRID: AB_2894838 |
| GRP78 (BiP) | R&D Systems | Cat# MAB4846; RRID: AB_2233235 |
| HA | Sigma | Cat# H-3663; RRID: AB_262051 |
| HSC70 | Santa Cruz | Cat# SC-7298; RRID: AB_627761 |
| HSP90 | Santa Cruz | Cat# SC-13119; RRID: AB_675659 |
| mCherry | Abcam | Cat# Ab167453; RRID: AB_2571870 |
| PDI (P4HB) | Abcam | Cat# Ab2792; RRID: AB_303304 |
| Procollagen I | Developmental Studies Hybridoma Bank | Cat# SP1.D8; RRID: AB_528438 |
| RPL5 | Abcam | Cat# Ab157099 |
| RPS2 | Bethyl | Cat# A303-794A; RRID: AB_11218192 |
| SAYSD1 | Abcam | Cat# Ab189918 |
| Sec61β | Tom Rapoport lab | N/A |
| UBE1 | Sigma | Cat# E–3152; RRID: AB_259297 |
| UFL1 | Bethyl | Cat# A303-456A; RRID: AB_10951658 |
| UFM1 | Abcam | Cat# Ab109305; RRID: AB_10864675 |
| VCP | Fitzgerald | Cat# 10R-P104A; RRID: AB_1287614 |
| Goat anti-Mouse IgG (AlexaFluor®680-conjugated) | ThermoFisher | Cat# A21058; RRID: AB_2535724 |
| Goat anti-Mouse IgG (HRP-conjugated) | Sigma | Cat# A4416; RRID: AB_258167 |
| Goat anti-Rabbit IgG (HRP-conjugated) | Sigma | Cat# A6154; RRID: AB_2766784 |
| Goat anti-Rabbit IgG (IRDye800-Conjugated) | Rockland | Cat# 611-132-003; RRID: AB_220151 |
| Goat anti-Rabbit IgG (AlexaFluor®568-conjugated) | ThermoFisher | Cat# A11011; RRID: AB_143157 |
| Goat anti-Mouse IgG (AlexaFluor®405-conjugated) | ThermoFisher | Cat# A48255; RRID: AB_2890536 |
| Goat anti-Mouse IgG (AlexaFluor®488-conjugated) | ThermoFisher | Cat# A32723; RRID: AB_2633275 |
|
| ||
| Bacterial and virus strains | ||
| TOP10 | ThermoFisher | C404010 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
| UFM1 | R&D Systems, Inc. | UL-522 |
| Ubiquitin | Sigma | U5507 |
| Acti-stain 555 phalloidin | Cytoskeleton, Inc. | PHDH1 |
| Anisomycin | Sigma | A9789 |
| L-Ascorbic acid | Sigma | L5960 |
| Bafilomycin A1 | LC Laboratories | B-1080 |
| cycloheximide | Sigma | C7698 |
|
| ||
| Critical commercial assays | ||
| Human Pro-Collagen I alpha 1 ELISA Kit | Abcam | Ab210966 |
|
| ||
| Deposited data | ||
| Raw and processed data | This paper | GEO: GSE222104 |
|
| ||
| Experimental models: Cell lines | ||
| 293T | ATCC | CRL-3216 |
| U2OS | ATCC | HTB-96 |
|
| ||
| Experimental models: Organisms/strains | ||
| UFM1-RNAi_1 | Bloomington Drosophila Stock Center | 36,796 |
| UFM1-RNAi_2 | Bloomington Drosophila Stock Center | 39,054 |
| gry (TRAPPC11)-RNAi | Bloomington Drosophila Stock Center | 64,038 |
| mCherry-RNAi | Bloomington Drosophila Stock Center | 35,785 |
| CG13663 sgRNA | Bloomington Drosophila Stock Center | 82,685 |
|
| ||
| Oligonucleotides | ||
| Silencer Select Negative Control No. 2 siRNA | Life Technologies | 4,390,847 |
| Silencer Select Pre-designed siRNA against UFM1 | Life Technologies | s28345 |
| Silencer Select Pre-designed siRNA against SAYSD1 | Life Technologies | s31482 |
| Silencer Select Pre-designed siRNA against SAYSD1 | Life Technologies | s31483 |
|
| ||
| Recombinant DNA | ||
| pLenti-CMV-GFP | Addgene | 17,446 |
| pLKO.1 puro | Addgene | 8453 |
| pET28-Mff(1–61)-PP-GST | Addgene | 73,042 |
| GeckoV2 sgRNA libraries | Addgene | 1,000,000,048 |
|
| ||
| Software and algorithms | ||
| Adobe Illustrator | Adobe | N/A |
| Adobe Photoshop | Adobe | N/A |
| Cutadapt | Open source | N/A |
| Cytoscape | Open source | N/A |
| FASTQC | Open source | N/A |
| GraphPad Prism | Dotmatics | N/A |
| ImageLab | BioRad | N/A |
| ImageJ | Open source | N/A |
| NIS Elements | Nikon | N/A |
| Zen | Zeiss | N/A |