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. Author manuscript; available in PMC: 2023 Jul 22.
Published in final edited form as: Circ Res. 2022 Jun 20;131(3):207–221. doi: 10.1161/CIRCRESAHA.121.320546

Figure 1. CKD induces myocardial SIRPα expression and release into serum.

Figure 1.

(A) Blood urea nitrogen (BUN) and creatinine were measured at 10–12 weeks after subtotal nephrectomy or sham operation in WT and SIRPα Mt mice with or without CKD. (B) At week 16 after subtotal nephrectomy, protein lysates of hearts were immunoblotted to detect SIRPα and GAPDH and representative immunoblots of averaged data (top panel) with relative densities of GAPDH (bottom panel) are shown. (C) Immunohistochemistry staining of antibody against SIRPα (arrows) from heart sections of WT and SIRPα Mt with or without CKD (scale bar =25 µm) and representative images of averaged data are shown. (D) Serum from Sham and CKD mice were immunoblotted for SIRPα and representative immunoblots of averaged data (top panel), with relative densities to total protein (bottom panel) are shown. (E) Serum was obtained from age-matched, healthy control subjects and patients with advanced CKD to detect circulating serum levels of SIRPα, representative immunoblots of averaged data (top panel), with relative densities to total protein (bottom panel) are shown. Statistical significance was calculated using one-way ANOVA with Bonferroni's multiple comparisons test (A) and unpaired two-tailed Studen's t-test (B, D-E). Values are means ± SEM. ** p<0.01, *** p<0.001, **** p<0.0001.