Fig. 6.

Inhibition of GSK-3β increases AAH protein expression: PNET2 cells were transfected with siRNA-GSK-3β or scrambled RNA sequences. 24 h later, cells were examined for a AAH, GSK-3β, and p85 (PI3K) immunoreactivities by Western blot analysis and (b–d) digital image quantification of the Western blot signals. Graphs depict the mean ± SEM levels of immunoreactivity. Inter-group statistical comparisons were made using Student t-tests and significant P-values are shown over the bars