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. 2023 Mar 10;14:30–49. doi: 10.18632/genesandcancer.231

Figure 4. VCP, a regulator of DDR, helps mediate DNA damage repair in PDAC.

Figure 4

(A) Representative images of immunofluorescence (IF) to monitor γH2AX expression (green) and nuclei (white) in PDAC cells after 24 hours of treatment with DMSO or CB-5083 at the indicated concentrations (nM). Scale bar, 75 μm. (B) Quantitation of relative integrated intensity of γH2AX per nucleus of cells shown in panel A. Each dot represents a nucleus; each bar indicates the mean of that treatment group. Statistical significance was calculated using one-way ANOVA and Kruskal-Wallis test relative to DMSO. **p < 0.01 and ****p < 0.0001. The total number of nuclei analyzed for each cell line and condition (DMSO, 250 nM CB-5083, 500 nM CB-5083) were: Pa01C (1460, 1143, 1495), Pa16C (545, 746, 592), and PANC-1 (962, 902, 588), respectively.