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. 2023 Feb 27;11:990711. doi: 10.3389/fcell.2023.990711

FIGURE 2.

FIGURE 2

E2F1-ac targets’ transcripts after ARRB1 modulation. (A,B) Expression of pro-apoptotic E2F1-ac target genes (Trp73, Casp3 and Casp7) in GCPs that had or had not undergone siArrb1 (black) or over-expression of Arrb1 (Arrb1-HA) (orange). (C,D) Expression of proliferative (grey) and epithelial mesenchymal transition (violet) E2f1-ac target genes (Cdc25a, Birc5, Tyms, Zeb1, Zeb2, Vim and Fn1) in GCPs that had or had not undergone siArrb1. Data represent means ± S.D., from at least three independent experiments; *p < 0.05; **p < 0.01; ***p < 0.001. (E) IHC staining for ARRB1 and P27 in representative mouse cerebellum sections at several differentiation stages (p2, p4, p7, p10, p15) (upper panels). Magnification ×10; insets ×40. Scale bar, 250 µm. CASPASE-3 and CDC25A indexes (expressed as percentage) calculated as DAB positive cells of total number of cells (bottom panels). Data represent means ± S.D., from at least three independent experiments; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.001 vs. p10. # p < 0.05; ## p < 0.01 vs. p15.