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. 2023 Feb 27;11:990711. doi: 10.3389/fcell.2023.990711

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Copyright © 2023 Abballe, Alfano, Antonacci, Cefalo, Cacchione, Del Baldo, Pezzullo, Po, Moretti, Mastronuzzi, De Smaele, Ferretti, Locatelli and Miele.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ARRB1-E2F1 complex direct regulates the expression of E2F1-ac pro-apoptotic targets. (A,B) qPCR-ChIP assay of ARRB1 in GCPs stimulated or not with Shh. Immunoprecipitation with IgG was performed as control. Eluted DNA was amplified by qPCR using primers specific for the regulatory region of the indicated genes. Actin and Gapdh (not shown) were used as endogenous non-enriched regions. qPCR data are presented as percentage of ChIP input controls. Data represent means ± S.D., from at least three independent experiments; *p < 0.05; **p < 0.01; ***p < 0.001.