FIG. 1.

Identification of incompatibility determinants (inc) of pTAV320. Only the restriction sites used to construct recombined plasmids are shown on the pTAV320 restriction map. The transcriptional orientations of the three open reading frames are indicated by arrows. The conserved intergenic sequence between repB and repC (igs) is enclosed in a box. The open boxes represent DNA restriction or PCR-amplified fragments of pTAV320 that were cloned into the pRK415 vector and tested for the presence of the inc regions; the designations of the pRK415 derivatives are on the right. For details concerning construction see Table 1. The lines joining the boxes indicate regions of pTAV320 removed by deletion. The smallest cis-acting regions of pTAV320 carrying identified incompatibility determinants are designated inc1 and inc2. A plus sign indicates incompatibility and a minus sign indicates compatibility of pTAV320 with derivatives of pRK415 introduced into P. versutus UW225(pTAV320).