FIG. 2.

Nucleotide sequences of the inc1 (A) and inc2 (B) regions of mini-replicon pTAV320. Several short repeated sequences identified in inc1 are indicated by different lines. Motif 1 and motif 2, the two motifs of inc1 sequences that are visibly conserved in the igs of repABC replicons, are indicated by boldface type and underlined. Two direct repeats of inc2, R1 and R2, are indicated by a black background. The region of inc2 amplified with primers INC10 and INC11 and used for EMSA is indicated by a thick line over the sequence. The palindromic sequences of the inc regions are indicated by arrows. The terminal fragments of the repB and repC genes are enclosed in boxes. The amino acid sequences of the proteins are given below the nucleotide sequences. The HindIII, PvuII, and BglII restriction sites important for the analysis are indicated. A presumptive ribosome binding site (rbs) is indicated. Coordinate numbers for the pTAV320 sequence are indicated on the right (accession number U60522).