Figure 4.

Neuron-specific SIRT3 deletion results in sex-specific effects on cellular metabolism. A, Representative image of in vitro extracellular acidification rate. B, Quantification of baseline glycolysis and (C) glycolytic reserve. n = 9-15/group (biological replicates), 10 technical replicates, across 9 total experiments. Data compared by two-way ANOVA followed by Sidak's multiple comparison test. Mitochondrial oxygen consumption compared by high resolution respirometry in the presence of CI and CII substrates and inhibitors in 12-month-old (D) female and (E) male mice (n = 6-10). *p < 0.05 (multiple t test). Error bars indicate mean ± SEM.