Table 1.

Sequence of donor DNA for generation of Oprm1-Cre knock-in rat

5′ arm of homology (nucleotides 63-98 are oprm1 exon 4)	CATCAATGTGCTCTCTAATGAGACCCCAGAACTCACTATCTTCACTCTTTCTCTTCTTTCAGCTAGAAAATCTGGAGGCAGAAACTGCTCCATTGCCC
Gly-Ser-Gly linker and T2A self-cleaving peptide sequence (lowercase)	GGTTCTGGCgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccct
iCre recombinase coding sequence	GTGCCCAAGAAGAAGAGGAAAGTCTCCAACCTGCTGACTGTGCACCAAAACCTGCCTGCCCTCCCTGTGGATGCCACCTCTGATGAAGTCAG GAAGAACCTGATGGACATGTTCAGGGACAGGCAGGCCTTCTCTGAACACACCTGGAAGATGCTCCTGTCTGTGTGCAGATCCTGGGCTG CCTGGTGCAAGCTGAACAACAGGAAATGGTTCCCTGCTGAACCTGAGGATGTGAGGGACTACCTCCTGTACCTGCAAGCCAGAGGCCT GGCTGTGAAGACCATCCAACAGCACCTGGGCCAGCTCAACATGCTGCACAGGAGATCTGGCCTGCCTCGCCCTTCTGACTCCAATGCTG TGTCCCTGGTGATGAGGAGAATCAGAAAGGAGAATGTGGATGCTGGGGAGAGAGCCAAGCAGGCCCTGGCCTTTGAACGCACTGACT TTGACCAAGTCAGATCCCTGATGGAGAACTCTGACAGATGCCAGGACATCAGGAACCTGGCCTTCCTGGGCATTGCCTACAACACCCTG CTGCGCATTGCCGAAATTGCCAGAATCAGAGTGAAGGACATCTCCCGCACCGATGGTGGGAGAATGCTGATCCACATTGGCAGGACCA AGACCCTGGTGTCCACAGCTGGTGTGGAGAAGGCCCTGTCCCTGGGGGTTACCAAGCTGGTGGAGAGATGGATCTCTGTGTCTGGTGT GGCTGATGACCCCAACAACTACCTGTTCTGCCGGGTCAGAAAGAATGGTGTGGCTGCCCCTTCTGCCACCTCCCAACTGTCCACCCGGG CCCTGGAAGGGATCTTTGAGGCCACCCACCGCCTGATCTATGGTGCCAAGGATGACTCTGGGCAGAGATACCTGGCCTGGTCTGGCCA CTCTGCCAGAGTGGGTGCTGCCAGGGACATGGCCAGGGCTGGTGTGTCCATCCCTGAAATCATGCAGGCTGGTGGCTGGACCAATGT GAACATTGTGATGAACTACATCAGAAACCTGGACTCTGAGACTGGGGCCATGGTGAGGCTGCTCGAGGATGGGGAC
Oprm1 termination codon and 3′ arm of homology from oprm1 exon 4	TAACTGGGTCTCACACCATCCAGACCCTCGCTAAGCTTAGAGGCCGCCATCTACGTGGAATCAGGTTGCTGTCAGGGTGTGTGGGAGGCTCTGGTTTCCTG