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. 2023 Mar 1;19(3):e1010656. doi: 10.1371/journal.pgen.1010656

Fig 3. DND1-GFP-lo cells express more phosphatidylserine on their outer membrane, as seen through Annexin V fluorescence.

Fig 3

A: Representative profiles of Annexin V fluorescence in E16.5 DND1-GFP-hi (yellow) and E16.5 DND1-GFP-lo (sky blue) populations. B: Geometric mean of Annexin V fluorescence in DND1-GFP-lo and DND1-GFP-hi cells. C: Representative fluorescence profile of DND1-GFP and Annexin V. Q1 (quadrant 1) = DND1-GFP-lo cells + Annexin V-positive. Q2 = DND1-GFP-hi cells + Annexin V-positive. Q3 = DND1-GFP-hi cells + Annexin V-negative. Q4 = DND1-GFP-lo cells + Annexin V-negative. D: Percent of DND1-GFP-lo cells and DND1-GFP-hi cells that are Annexin V-positive. E: Percent of total Annexin V-positive and negative cells that are DND1-GFP-lo cells. A-E: Technical replicates = 4, biological replicates > 2 fetal testes. P value: ****<0.0001, *** = 0.0008, ** = 0.0014.