A: PANTHER analysis of significant differentially expressed genes in E16.5 and E18.5 DND1-GFP-lo cells, and E16.5 and E18.5 DND1-GFP-hi cells. Results displayed as protein class categories by dataset and graphed with the number of genes in each category corresponding to the size of the circle. Protein classes distinguishing DND1-GFP-lo cells at both stages include “Defense and Immunity”, “Transmembrane Signal Receptor” and “Intercellular Signal Molecule” (noted with black vertical line). Protein classes distinguishing DND1-GFP-hi cells at both stages include “Chaperone”, “Chromatin Binding/Regulation”, “Nucleic Acid Metabolism”, and “Translational” (noted with black vertical line). B: Heat map of chromatin / epigenetic regulators showing: L2FC in DND1-GFP-hi vs DND1-GFP-lo cells from hiloRNA-seq (E16.5, E18.5).