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. 2023 Feb 24;14:1108197. doi: 10.3389/fmicb.2023.1108197

Figure 1.

Figure 1

Comparison of alpha diversity indices based on post-weaning diarrhea (PWD) status. Groups were compared at post-natal day (PND) 8 (early lactation, healthy: n = 20 pigs, PWD: n = 24 pigs), at PND 27 (late lactation, healthy: n = 20 pigs, PWD: n = 23 pigs), and PND 33 (7 days after weaning, healthy: n = 18 pigs, PWD: n = 25 pigs). Shannon and Simpson indices and number of observed zOTUs were based on a rarefied zOTU table generated from 16S rRNA V3 high throughput amplicon sequencing. Graphs show boxplots with medians marked with bold line. Statistical differences between groups were assessed with linear mixed models. No significant group differences were found for PWD status at any time point. At PND 33, the covariable body weight at PND 33 was positively associated with increased Shannon diversity (effect size: 0.13, 95% CI: [0.068; 0.208], p = 0.0002), Simpson’s diversity (effect size: 0.001, 95% CI: [−0.00012; 0.002], p = 0.07), and number of observed zOTUs (effect size: 86.33, 95% CI: [46.27; 126.80], p = 0.00009).