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. 2023 Jan 27;147(11):881–896. doi: 10.1161/CIRCULATIONAHA.122.061955

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© 2023 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 1. — BioChIP of P300 and 7 key cardiac transcription factors in atria and ventricles. A, aCM- and vCM-biased genes. Heatmap shows genes differentially expressed (P_adj<0.05 and | log₂ fold-change | >0.58) between purified P0 aCMs and vCMs, as determined by RNA-sequencing. B, Gene ontology terms enriched among genes upregulated in aCMs or vCMs. C, Strategy for bioChIP-sequencing. Biotin acceptor peptide (bio) was knocked in the C terminus of target genes and biotinylated by BirA expressed from the Rosa26 locus. D, Heatmap of TF or P300-bound regions in atria and ventricles. Each row of each heatmap shows a ±3-kb region that is reproducibly bound by the indicated factor, centered on the bioChIP-sequencing peak center. Brown and blue lines indicate atrial and ventricular regions. Regions with chamber-selective occupancy are shown in the top and bottom groups, and the middle group shows shared regions. The number of regions in each group is shown to the left. E, Enrichment of indicated TF motif (rows) in the top 1000 ChIP-sequencing peaks of the indicated TF (columns) in atria or ventricles. F, Relationship of regions occupied by P300 and by ≥5 TFs (MTF) in atria and ventricles. aCM indicates atrial cardiomyocyte; MTF, multiple transcription factors; TF, transcription factor; and vCM, ventricular cardiomyocyte.