Table 1. Multivariate linear models of the dependence of calcium peak frequency (PF) on genotype.

Listed linear models were fit to peak frequencies of calcium transients measured from 87 control and 98 TTX-treated slices each from separate animals that differed in their genotype (null, heterozygote or wild-type) for each of the Hlf, Tef, and Dbp genes. The data (see attached file) includes the 88 experiments shown in Figure 2 and Figure 2—figure supplement 1 (WT and TKO) as well as 97 additional experiments (intermediate genotypes). Regressions were performed using the lm function in R where the variables Hlf, Tef, and Dbp represent the numbers of alleles of each gene (0, 1, or 2) and the variable (Hlf + Tef) represented the sum of the number of Hlf and Tef alleles. The column p > F reports the significance of each model assessed from an F test. For models that accounted for >10% of the variance in the data (adj R² > 0.1; bold values), the intercept (b₀) and coefficients (b1…) are listed, along with the 790 probability that the coefficient is nonzero (t test).

	Model (TTX)	Adj R2	Pr > F	b0 (Pr > t)	b1 (Pr > t)	b2 (Pr > t)	b3 (Pr > t)
1	PF = b0 + b1(Hlf) + b2(Tef) + b3(Dbp)	0.284	2E−07	0.457 (<2E−16)	–0.055 (0.003)	–0.068 (4E−4)	0.006 (0.71)
2	PF = b0 + b1(Hlf)	0.198	4E−06	0.423 (<2E−16)	–0.081 (4E−6)
3	PF = b0 + b1(Tef)	0.229	4E−07	0.437 (<2E−16)	–0.088 (4E−7)
4	PF = b0 + b1(Dbp)	0.073	0.007
5	PF = b0+b1(Hlf) + b2(Tef)	0.291	3E−08	0.458 (<2E−16)	–0.052 (0.003)	–0.066 (2E−4)
6	PF = b0 + b1(Hlf + Tef)	0.296	4E−09	0.485 (<2E−16)	–0.059 (4E−9)
	Model (Ctrl)
7	PF = b0 + b1(Hlf) + b2(Tef) + b3(Dbp)	0.044	0.08
8	PF = b0 + b1(Hlf)	–0.01	0.84
9	PF = b0 + b1(Tef)	0.005	0.52
10	PF = b0 + b1(Dbp)	0.063	0.05
11	PF = b0 + b1(Hlf) + b2(Tef)	–0.01	0.62
12	PF = b0 + b1(Hlf + Tef)	–0.01	0.80