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. 2023 Mar 13;12:e75906. doi: 10.7554/eLife.75906

Figure 8. Furrow collagen inactivation provokes extrusion of membrane and cytoplasmic contents into the cuticle.

Figure 8.

(A) TEM images of dpy-2 (left) and dpy-7 (right) young adult mutant worms reveal the presence of membranous organelles (*) and cytoplasmic content, including ribosome-like particles (#), between the cuticle and the plasma membrane; epidermis is pseudo-coloured in beige. Scale bar, 500 nm. (B) Confocal images of wild-type (left) and dpy-3 mutant (right) young adult worms expressing ROL-6::mScarlet and VHA-5::GFP, CAAX::GFP or PH-PLC1δ::GFP, one confocal plane was selected at the level of the cuticle using the ROL-6::mScarlet. Scale bar, 10 µm. (C) Quantification of the percentage of worm presenting the abnormal presence of GFP extrusion at the level of the cuticle for the different markers; number of worms observed is noted in parenthesis.