TABLE 2.
Plasmids used
| Plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| pUC18 | Cloning vector (Apr) | Amersham Pharmacia |
| pSR1 | pBluescript KS II(+) with 2,810-bp amplicon containing minCDENg | 30 |
| pSR3 | pUC18 containing minDNg under lac promoter | This study |
| pJSDcat | pSR1 with cat inserted into minDNg | This study |
| pET30a | Cloning vector (Kmr) to produce N-terminal fusions to 6×-His tag | Novagen |
| pJSHD2 | pET30a containing 6×-His-minDNg | This study |
| pFP10 | Gonococcal shuttle vector (Cmr) | 26 |
| pFP20 | pFP10 modified with kanamycin resistance cassette and additional restriction sites | This study |
| pFP22 | pFP20 containing minCNg and 503-bp upstream region of the minCNg start codon | This study |
| pFP23 | pFP20 containing minDNg and 714-bp upstream region of the minDNg start codon | This study |
| pFP25 | pFP20 containing minCDNg and 503-bp upstream region of the minCNg start codon | This study |
| pGBT9 | Yeast two-hybrid plasmid encoding GAL4 DNA-BD (Amr) | Clontech |
| pGBT9minD | pGBT9 encoding GAL4 DNA-BD fused to MinDNg | This study |
| pGAD424 | Yeast two-hybrid plasmid encoding GAL4-AD (Amr) | Clontech |
| pGADminD | pGAD424 encoding GAL4-AD fused to MinDNg | This study |
| pSRBD-D | pGBT9 encoding GAL4 DNA-BD fused to MinDEc | This study |
| pSRAD-D | pGAD424 encoding GAL4-AD fused to MinDEc | This study |
| pVA3 | Yeast two-hybrid plasmid encoding murine p53, used for positive control interaction with pTD1 | Clontech |
| pLAM5′ | Yeast two-hybrid plasmid encoding human laminC, used for negative control interaction with pTD1 | Clontech |
| pTD1 | Yeast two-hybrid plasmid encoding SV40 large T antigen, used for positive control interaction with pVA3 | Clontech |