TABLE 3.
Yeast two-hybrid analysis of MinD self-interactions
| Fusion to GAL4 DNA-BD | Fusion to GAL4-AD | Interaction detected | β-Galactosidase activity (Miller units)a |
|---|---|---|---|
| MinDNg | MinDNg | Yes | 12.3 ± 3.4 |
| MinDEc | MinDEc | Yes | 4.4 ± 0.9 |
| MinDNg | MinDEc | Yes | 14.3 ± 0.6 |
| MinDEc | MinDNg | Yes | 28.8 ± 7.8 |
| p53b | SV40 large T antigenb | Yes | 27.2 ± 6.0 |
| LaminCc | SV40 large T antigenc | No | 0.1 ± 0.1 |
a
One unit equals the amount of β-galactosidase which hydrolyzes 1 μmol of ONPG per min per cell.
b
Positive control interaction.
c
Negative control.