FIG. 4.

Leukotriene B4 (LTB-4) production is enhanced in EPEC-infected cells and inhibited by lipoxygenase inhibitor NDGA. Lane 1, lysates of HEp-2 cells incubated with DMSO (1 μl/ml); lane 2, lysate derived from cells incubated for 1 h with NDGA (35 μM); lane 3, cell lysates following incubation with NDGA (75 μM) for 1 h; lane 4, lysates obtained from cells incubated with EPEC strain E2348/69 (10⁸) for 3 h; lane 5, cell lysates from HEp-2 cells preincubated for 1 h with NDGA (35 μM) and then infected with EPEC; lane 6, lysates from cells preincubated with NDGA (75 μM) for 1 h and then infected with EPEC. Results are the mean values of four separate experiments each run in duplicate. ∗∗∗, P < 0.01 (ANOVA).