Figure 1.

Glymphatic flow assessment by cisterna magna injections. A shows typical 100-µm coronal brain section fluorescence after injections of ovalbumin conjugated to Alexa Fluor 555 and cadaverine conjugated to Alexa Fluor 488. B shows quantitative evaluation of protein uptake in control mice and mice studied 2- or 24-h postexposure to 790 kPa air pressure for 2 h. Values are means ± SD (for control, 3 male and 3 female mice; for 2-h post-air pressure group, 4 male and 3 female mice; for 24-hour post-air pressure group, 3 male and 3 female mice). Evaluations were performed on 8 brain sections/mouse; individual data points are also shown. WTDeco, wild-type mouse subjected to pressure/decompression.