Figure 4.

Neuroinflammation in postpressure mouse brains. A shows typical immunohistochemical images with 20-μm scale bars in lower right of each image. The table below the figure shows means ± SD (n = 6 mice, with 4–6 brain slices quantified per mouse) number of cells in brain slices that demonstrated IBA-1 concurrent with DAPI nuclear staining and triple staining with GFAP, p65 subunit of NF-κB, and DAPI in control mice, 2 h postdecompression mice and mice intravenously injected with 60,000 F-actin-positive MPs from mice euthanized 2 h postdecompression. B shows a representative brain homogenate Western blot where numbers below each band indicate means ± SD (n = 4–9 mouse brains per lane) band densities relative to β-actin of control samples from replicate studies. Blots for each protein are contiguous, no cuts/insertions were made. Molecular weight markers for blots are shown on the right margin. Numbers with an asterisk indicate values statistically significant from control (P < 0.05 ANOVA). Blots were probed for the p65 subunit of NF-κB, serine 536 phosphorylated p65 NF-κB (labeled phospho-NF-κB), Ly6G, MPO, CD36, and AQP4. Lanes reflect control mice, those euthanized 2 h postdecompression, neutropenic mice euthanized 2 h postdecompression (note that control neutropenic mice exhibited no statistically significant differences from normal controls but data not shown), naïve mice injected with 60,000 F-actin-positive MPs from mice euthanized 2 h postdecompression, naïve mice injected with 60,000 F-actin-negative MPs from mice euthanized 2 h postdecompression, neutropenic mice injected with 60,000 F-actin-positive MPs from mice euthanized 2 h postdecompression, naïve mice injected with 60,000 cervical node MPs from mice euthanized 2 h postdecompression, naïve mice injected with 60,000 F-actin-positive MPs from mice postdecompression that had first been incubated with antibody to TSP-1. MP, microparticle; MPO, myeloperoxidase; TSP, thrombospondin-1.