Extended Data Fig. 2. The KD effectiveness of each ASO against MERVL in preimplantation embryos.
(a) Representative images of smFISH for MERVL RNA (green) with DAPI counterstain (gray) in two-cell stage embryos from each experimental condition, from 3 independent experiments. Zygotes were injected with scrambled ASO, MERVL ASOs or the corresponding SOs. Scale bar: 20 µm. (b) Representative phase-contrast images of 4.5 dpc blastocysts from each experimental condition, from 2 independent experiments. Scale bar: 100 µm. (c) Percentage of embryos by developmental stage at 4.5 dpc in each experimental condition. The number of embryos in each experimental condition is shown in the bottom. Data for panels in c are available as source data.