Figure 2.

Durable humoral immune response elicited by Ad5-GnGcopt in BALB/c mice. BALB/c mice (n = 8) were intramuscularly immunized with a single dose of Ad5-GnGcopt (10⁶, 10⁷, or 10⁸ IFU) or placebo. Sera were collected at 2, 4, 6, 8, 10, 13, and 18 weeks after vaccination. RVFV (A) Gn- and (B) Gc-specific IgG antibody titer was determined by ELISA. (C,D) Sera from the high-dose group collected on day 28 after immunization determined (C) Gn- and (D) Gc-specific IgG subclasses. (E) Serum samples collected 4, 10, and 18 weeks after vaccination were subjected to neutralizing antibody titers detection. Data are indicated as mean ± SEM. One-way ANOVA with multiple comparison tests calculated p values. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.