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. 2023 Mar 13;14:1384. doi: 10.1038/s41467-023-36988-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Box-and-whisker plots (a–c) show estimated average gene length (AGL), percent guanine and cytosine (% GC), and number of unique genes per megabase pair (Mbp) of coding sequence length (UGPCL) in metagenomic assemblies from the global Malaspina expedition (n = 81). Depth and number of samples analyzed are as follows: the epipelagic (EPI, ~3–200 m; n = 23), mesopelagic (MES, > 200–1000 m; n = 32), and bathypelagic (BAT, > 1000–4000 m; n = 26). Boxplots show median as horizontal lines and interquartile ranges as boxes (whiskers extend to a maximum of 1.5 times the interquartile range). Mean values are shown as white colored diamonds. Values at the top indicate the adjusted significant P-values of the unpaired two-sided Wilcoxon test with Benjamini-Hochberg correction. d Relationship between genetic (AGL, %GC, and UGPCL) and environmental (temperature, depth, and salinity) factors in the sampled global ocean microbiomes (n = 81). Values indicate Spearman correlations coefficient (one-tailed). Asterisks mark non-significant relationships (p > 0.01). Source data are provided as a Source Data file.