Table 3.

Comparison of crypt depth measures between Olfm4-stained crypts and hematoxylin stained crypts.¹

Day postinfection	Stain	Uninfected (μm)	Infected (μm)
0	Olfm4	95.8	70.8
	Hematoxylin	121.4	91.4
3	Olfm4	78.6	146.4
	Hematoxylin	103.8	168.0
5	Olfm4	86.0	172.5
	Hematoxylin	101.1	231.8
7	Olfm4	130.6	223.7
	Hematoxylin	145.3	256.6
10	Olfm4	81.5	146.0
	Hematoxylin	109.8	182.6
14	Olfm4	77.1	128.7
	Hematoxylin	105.2	129.5
SEM2		69.3	52.4
Welch's unequal variance test		Probabilities
Stain		0.048
Infection status		< 0.0001
Day postinfection		0.0027

¹Measures following Olfm4 in situ hybridization using RNAscope 2.5 HD kit (Brown) or Hematoxylin staining in broiler chickens that were infected at 21 d of age with 100,000 Eimeria acervulina oocysts (Infected) or sham infected with sterile water (Uninfected) and sampled at 0 (3 h postinfection), 3, 5, 7, 10, and 14 d post infection (dpi). Measurements were taken at 40 X magnification (n = 6) using ImageJ.

²SEM = pooled standard error.