Fig. 2. Adipocyte-specific knockout of Ythdf1 predisposes mice to HFD-induced obesity.

a Immunoblotting of adipose tissues from Y1^CTL and Y1^cKO mice. b Schematic of the mouse treatment regimen. c–n The Y1^CTL and Y1^cKO littermates were fed with HFD. c Daily food intake by Y1^CTL and Y1^cKO mice. d Gross view of Y1^CTL and Y1^cKO mice. Scale bar, 0.5 cm. e Body weights of Y1^CTL and Y1^cKO mice. f Gross view and weights of iWAT, eWAT, and BAT from Y1^CTL and Y1^cKO mice. Scale bar, 0.5 cm. g H&E staining of iWAT eWAT, and BAT from Y1^CTL and Y1^cKO mice. Scale bar, 50 μm. The red line indicated the average size. No adjustments. h–j O₂ consumption h, CO₂ generation i, and energy heat generation j of Y1^CTL and Y1^cKO mice. White and gray areas in the graphs indicate day and night, respectively. k Rectal temperature in Y1^CTL and Y1^cKO mice. l Serum concentrations of TG, CHO, LDL, and HDL in Y1^CTL and Y1^cKO mice fed with HFD. m, n glucose tolerance m and Insulin tolerance n fed with HFD. Data in c, e–n were presented as mean ± SEM (n = 4 biologically independent mice). ns, not significant, *P < 0.05, **P < 0.01, ****P < 0.0001, two-sided t-test. Source data are provided as a Source Data file.