Fig. 6. Spatial distribution of immune cell subsets in allografted lungs.

BALB/c lungs were transplanted as in Fig. 1a into C57BL/6 recipients pre-treated as indicated. a Distance of Foxp3⁺ T (red lines), Foxp3^– conventional CD4⁺ T (black lines), and CD8⁺ T cells (green lines) from nearest SMA⁺ bronchus based on immunofluorescence analysis in grafted lungs of representative PBS- (top panels) and IL-2cx-treated mice (bottom panels). b Quantification of Foxp3⁺CD4⁺ Treg (left graph), Foxp3^– conventional CD4⁺ T (middle graph), and CD8⁺ T cells (right graph) based on immunofluorescence analysis of whole lungs. c Quantification of Foxp3⁺CD4⁺ Treg, Foxp3^– conventional CD4⁺, and CD8⁺ T cells in whole lungs, represented as stacked bar graphs. d Distance of B220⁺ B (red lines) and CD11c⁺ myeloid cells (green lines) from nearest SMA⁺ bronchus based on immunofluorescence analysis in grafted lungs of PBS- and IL-2cx-treated mice on day 15 after transplantation. e Quantification of B220⁺ B (left graph) and CD11c⁺ myeloid cells (right graph) based on immunofluorescence analysis. f Quantification of the areas under the curve (AUC) of normalized cell counts of Foxp3⁺CD4⁺ Treg, Foxp3^– conventional CD4⁺, and CD8⁺ T cells within 150 μm from the nearest SMA⁺ bronchus. g Ratios of CD4⁺Foxp3⁺ Treg over CD4⁺Foxp3^– T cells and of CD4⁺Foxp3⁺ Treg over CD8⁺ T cells in total lungs. h Quantification of cells neighboring T cells, shown in overview (left graph) and as representative examples in indicated conditions and at indicated timepoints. Data are presented as mean ± SD of n ≥ 3 mice of 2–3 independent experiments. Significance of difference in (b, e–h) was calculated using two-sided Student’s t test with calculated p values shown.