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. 2023 Mar 13;8:15. doi: 10.1038/s41536-023-00288-1

Fig. 3. Human BMSC survival and proliferation.

Fig. 3

a Reconstruction of graft sections under fluorescence microscopy (layer subdivision in yellow); b Example of immunostaining of human nuclei (HuNu, in red), proliferating cells (Ki67, also in the nucleus, in green) and all nuclei (DAPI, in blue) of constructs (representative picture of the outer layer of TG-VEGF 0.1 μg/ml condition) after 1 week of in vivo implantation; c Immunostaining of human nuclei (HuNu, in red), dying cells (CAS3, also in the nucleus, in green) and all nuclei (DAPI, in blue) of constructs (representative picture of the middle layer of TG-VEGF 10 μg/ml condition) after 1 week of in vivo implantation d Quantification of proliferating human cells (%) in each layer. e Quantification of dying human cells (%) in each layer. Values are expressed as mean ± s.e.m.; *p < 0.05, **p < 0.01, ***p < 0.001. N = 4–6. Scale bars (a) = 500 µm; (b, c) = 50 µm.