Fig. 4. Long term vascularization of osteogenic constructs.
a, c Immunostaining of endothelium (CD31, in red) and nuclei (DAPI, in blue) of constructs, with TG-VEGF doses ranging from 0 to 100 µg/ml, after 4 (a) and 8 (c) weeks of in vivo implantation. b, d Quantification of vessel length density (VLD), expressed as millimeters of vessel length per square millimeter of tissue area (mm/mm2) after 4 (b) and 8 (d) weeks of in vivo implantation. Values are expressed as mean ± s.e.m.; *p < 0.05, ***p < 0.001. N = 4–6. Scale bars = 100 μm.