Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 21;34(3):ar18. doi: 10.1091/mbc.E22-10-0473

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Palicharla et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.

PMC Copyright notice

FIGURE 4: — TULP3 traffics ARL13B in a PI(4,5)P₂- independent manner unlike GPCR cargoes. (A) Schematic showing mutants. (B) Tulp3 ko IMCD3 line #89 stably expressing ^HAINPP5E and the indicated ^LAPTULP3 variants were grown to confluence and serum starved for 36 h before fixation. Cells were immunostained for ARL13B, HA (INPP5E), GPR161, GFP (TULP3), and acetylated tubulin (AcTUB) and counterstained for DNA. *, cytokinetic bridge. (C) Cilia in GFP(TULP3)-expressing cells were counted from three experiments, n > 200/condition. Data represent mean ± SD. (D, E) Violin plot shows mean ciliary intensities from B. n = 50 cilia/condition (ARL13B) and 30 cilia/condition (^HAINPP5E). (F) Cilia lengths were measured in B by acetylated tubulin immunostaining (50 cilia each). Scale, 5 μm. ****, p < 0.0001; **, p < 0.01; *, p < 0.05; ns, not significant. Arrows and arrowheads indicate cilia positive and negative for the indicated proteins, respectively.