FIGURE 6:

ARL13B domains required for TULP3 interactions. (A) Schematic representation of different ARL13B truncations used summarizing TULP3 binding. Coiled-coiled domain, CC; proline-rich domain, PR. (B) Binding between GST or ^GSTTULP3 bound to glutathione sepharose beads and IVT Myc-tagged truncations of ARL13B as in Figure 5D, except incubated in LAP150N at room temperature for 1 h. (C) Alignment of N-terminus region of ARL13B preceding the GTPase domain and mutants. ARL13B protein IDs: Hs, Homo sapiens NP_001167621.1; Mm, Mus musculus NP_080853.3; Rn, Rattus norvegicus NP_001100571.1; Dr, Danio rerio NP_775379.1; Cr, Chlamydomonas reinhardtii A8INQ0.1. Palm, conserved palmitoylation motif (Ren et al., 2008; Weng et al., 2017). Helix for the human protein drawn using HeliQuest (Gautier et al., 2008). Arrow, hydrophobic moment; N/C, N/C-terminal. (D) Binding as in B using ARL13B mutants described in C. (B, D) ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05. ns, not significant. See also Supplemental Figure S4.