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. 2023 Feb 9;299(3):102995. doi: 10.1016/j.jbc.2023.102995

Figure 1.

Figure 1

APP overexpression restricts poly(GR) expression in Drosophila muscle.A, Western blot results showing Flag-(GR)80 expression in the presence of APP and Aβ-42. The 6E10 antibody is detecting APP and Aβ-42. Bar graph shows quantification of the relative expression of (GR)80. (∗∗∗p < 0.001; ∗∗p < 0.01) B, immunoblot results showing effect of APP-C99 coexpression on Flag-(GR)80 level in fly thoracic muscle extracts. Actin serves as loading control. Bar graph shows quantification of the relative expression of (GR)80 (∗∗∗p < 0.001). C, immunostaining of Flag-(GR)80 with Flag antibody and APP/Aβ-42 with 6E10 antibody in Mhc>Flag-(GR)80 flies coexpressing APP or Aβ-42. The scale bars represent 8 μm. D, immunostaining of Flag-(GA)80 and APP with Flag and 6E10 antibody, respectively, in Mhc>Flag-(GA)80 flies coexpressing APP. The scale bars represent 10 μm. APP, amyloid precursor protein.