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. 2022 Sep 8;3:945768. doi: 10.3389/fcdhc.2022.945768

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Copyright © 2022 Sugino, Mandala, Janssen, Gurung, Trammell, Day, Brush, Papin, Dyer, Agbaga, Friedman, Castillo-Castrejon, Jonscher and Myers

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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WD exposure increases monocyte infiltration of the placenta and induces fetal hepatic steatosis and fibrosis. Representative images for immunofluorescence in placenta tissue and quantitation (A). Red arrows point to MAC387-labeled macrophages (green). Blue staining - DAPI. (B) mRNA expression of cytokines in placenta using qPCR. ACTB was used for reference. Representative images of histological analysis of fetal liver tissue (C) with H&E, picrosirius red (PSR), and LipidSpot, taken at 100 um. Fetal liver triglycerides (TG) (D) and mRNA expression analysis using qPCR (E) with RPS9 used for normalization. n = 4-6 CD and n = 5 WD. Unpaired 2-tailed Student’s t test was used to test significance. *P < 0.05, ****P < 0.0001.