Table 1.
Associations of maternal metabolic measurements compared to maternal microbiota and placental miRNA.
| Microbiota | Placenta | |||||||
|---|---|---|---|---|---|---|---|---|
| Taxa (Genus or Family) | r2 | P value | FDR | miRNA | r2 | P value | FDR | |
| Dam metabolism | ||||||||
| Body wt | Clostridium | +0.01 | 0.0374 | 0.5478 | ||||
| Oscillospira | +0.32 | 0.0498 | 0.5729 | |||||
| Unclassified Lachnospiraceae | +0.41 | 0.0040 | 0.1300 | |||||
| SSF | miR-21-5p | -0.34 | 0.0340 | 0.1831 | ||||
| Dam lipid metabolism | ||||||||
| TGs | Unclassified Bacteroidales | +0.32 | 0.0029 | 0.1168 | miR-183-5p | -0.50 | 0.0129 | 0.1405 |
| miR-182-5p | -0.57 | 0.0068 | 0.1368 | |||||
| HDL | Unclassified Rickettsiales | +0.43 | <0.0001 | 0.0037 | miR-204-5p:sex | M: -0.56; F: 0.27 | 0.0355 | 0.1834 |
| Unclassified Verrucomicrobia | +0.38 | 0.0014 | 0.0342 | miR-21-5p:sex | M: -0.70; F: -0.34 | 0.0215 | 0.1565 | |
| Unclassified Bacteroidales | +0.18 | 0.0021 | 0.1156 | |||||
| Catenibacterium | +0.29 | 0.0161 | 0.3247 | |||||
| Succinivibrio | +0.25 | 0.0070 | 0.1890 | |||||
| LDL/VLDL | Unclassified Ruminococcaceae | +0.22 | 0.0496 | 0.5729 | miR-183-5p | -0.55 | 0.0084 | 0.1368 |
| Unclassified Rickettsiales | +0.05 | 0.0242 | 0.4329 | miR-199a-5p:sex | M: -0.77; F: 0 | 0.0437 | 0.2007 | |
| Treponema | -0.07 | 0.0122 | 0.2813 | |||||
| Dam inflammation | ||||||||
| CRP | Paraprevotellaceae | +0.49 | 0.0035 | 0.0632 | ||||
| Placenta | ||||||||
| Weight | Desulfovibrionaceae | +0.32 | 0.0012 | 0.0342 | ||||
| Methanobrevibacter | +0.11 | 0.0413 | 0.5547 | |||||
Maternal measures were compared to maternal gut microbiota abundances using negative binomial models. Maternal measures were compared to placental miRNAs with ANOVA. Both comparisons utilized LASSO regularization for variable selection.
CRP, C-reactive protein; HDL, high-density lipoprotein; LDL, low-density lipoprotein; SSF, sum of skin folds; TG, triglyceride; VLDL, very low-density lipoprotein; wt, weight.