TABLE 1.
Strains and plasmids in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| VH1000 | RLG3499 = MG1655 lacZ lacI pyrE+ | 19 |
| RJ1617 | MC1000 fis::kan-767 | 27 |
| RLG3669 | BL21 (DE3) pLysS | Novagen |
| λ system I lysogens | ||
| RLG4297 | VH1000 λ rrnB P1 (−88 to +1)-lacZ | This work |
| RLG4787 | VH1000 λ rrnE P1 (−89 to +1)-lacZ | This work |
| RLG4788 | VH1000 λ rrnE P1 (−209 to +1)-lacZ | This work |
| RLG4789 | VH1000 λ rrnE P1 (−61 to +1)-lacZ | This work |
| RLG4999 | VH1000 λ M13 polylinker-lacZ | This work |
| RLG5940 | RLG4999 fis::kan-767 | This work |
| RLG5949 | VH1000 λ rrnB P1 (−154 to +1)-lacZ | This work |
| RLG5950 | VH1000 λ rrnABC P1 (−61 to +1)-lacZ | This work |
| RLG6150 | VH1000 λ rrnG P1 (−199 to +1)-lacZ | This work |
| RLG6152 | VH1000 λ rrnH P1 (−205 to +1)-lacZ | This work |
| RLG6153 | VH1000 λ rrnH P1 (−62 to +1)-lacZ | This work |
| RLG6360 | VH1000 λ rrnG P1 (−61 to +1)-lacZ | This work |
| RLG6361 | RLG4297 fis::kan-767 | This work |
| RLG6362 | RLG5949 fis::kan-767 | This work |
| RLG6363 | RLG5950 fis::kan-767 | This work |
| RLG6369 | RLG6150 fis::kan-767 | This work |
| RLG6370 | RLG6152 fis::kan-767 | This work |
| RLG6371 | RLG6153 fis::kan-767 | This work |
| RLG6372 | RLG6360 fis::kan-767 | This work |
| RLG6373 | RLG6390 fis::kan-767 | This work |
| RLG6374 | RLG6392 fis::kan-767 | This work |
| RLG6375 | RLG6504 fis::kan-767 | This work |
| RLG6376 | RLG6394 fis::kan-767 | This work |
| RLG6377 | RLG6505 fis::kan-767 | This work |
| RLG6378 | RLG6396 fis::kan-767 | This work |
| RLG6379 | RLG6397 fis::kan-767 | This work |
| RLG6380 | RLG6398 fis::kan-767 | This work |
| RLG6381 | RLG6368 fis::kan-767 | This work |
| RLG6390 | VH1000 λ rrnG P1 (−86 to +1)-lacZ | This work |
| RLG6392 | VH1000 λ rrnH P1 (−82 to +1)-lacZ | This work |
| RLG6394 | VH1000 λ rrnA P1 (−199 to +1)-lacZ | This work |
| RLG6396 | VH1000 λ rrnC P1 (−201 to +1)-lacZ | This work |
| RLG6397 | VH1000 λ rrnD P1 (−81 to +1)-lacZ | This work |
| RLG6398 | VH1000 λ rrnD P1 (−198 to +1)-lacZ | This work |
| RLG6503 | VH1000 λ rrnD P1 (−60 to −42)-rrnB P1 (−41 to +1)-lacZ | This work |
| RLG6504 | VH1000 λ rrnA P1 (−81 to +1)-lacZ | This work |
| RLG6505 | VH1000 λ rrnC P1 (−81 to +1)-lacZ | This work |
| RLG6512 | VH1000 λ rrnABC P1 (−61 to −42)-rrnD P1 (−41 to +1)-lacZ | This work |
| RLG6513 | VH1000 λ rrnD P1 (−61 to +1)-lacZ | This work |
| RLG6516 | VH1000 λ rrnB P1 (−154 to −62)-rrnD P1 (−61 to +1)-lacZ | This work |
| RLG6517 | VH1000 λ rrnD P1 (−198 to −62)-rrnABC P1 (−61 to +1)-lacZ | This work |
| RLG6522 | VH1000 λ rrnE P1 (−209 to −62)-rrnABC P1 (−61 to +1)-lacZ | This work |
| RLG6523 | VH1000 λ rrnE P1 (−88 to −62)-rrnABC P1 (−61 to +1)-lacZ | This work |
| RLG6524 | VH1000 λ rrnB P1 (−154 to −62)-rrnE P1 (−61 to +1)-lacZ | This work |
| RLG6525 | VH1000 λ rrnB P1 (−88 to −62)-rrnE P1 (−61 to +1)-lacZ | This work |
| RLG6527 | RLG6513 fis::kan-767 | This work |
| RLG6528 | VH1000 λ rrnB P1 (−82 to +1)-lacZ | This work |
| λ system II lysogens | ||
| RLG6352 | VH1000 λ rrnE P1 “CA” (−41/+1)-lacZ | This work |
| RLG6353 | VH1000 λ rrnH P1 “CA” (−41/+1)-lacZ | This work |
| RLG6354 | VH1000 λ rrnG P1 “CA” (−41/+1)-lacZ | This work |
| RLG6358 | VH1000 λ rrnABC P1 “CA” (−41/+1)-lacZ | This work |
| RLG6502 | VH1000 λ rrnD P1 “CA” (−41/+1)-lacZ | This work |
| Plasmids | ||
| pRJ1077 | pET11a with fis | 39 |
| pRLG770 | Vector (no insert) | 46 |
| pRLG5943 | rrnB P1 (−154 to +1) in pRLG770 | This work |
| pRLG4782 | rrnE P1 (−209 to +1) in pRLG770 | This work |
| pRLG5945 | rrnG P1 (−199 to +1) in pRLG770 | This work |
| pRLG5947 | rrnH P1 (−205 to +1) in pRLG770 | This work |
| pRLG6385 | rrnA P1 (−199 to +1) in pRLG770 | This work |
| pRLG6387 | rrnC P1 (−201 to +1) in pRLG770 | This work |
| pRLG6398 | rrnD P1 (−198 to +1) in pRLG770 | This work |