Table 2.
TLC identification of Wuzi Yanzong prescription
| Dosage form | Control ingredients and medicinal materials | Developing solvent | Reference |
|---|---|---|---|
| Wuzi Yanzong Wan | Scopoletin | Petroleum ether (30-60)-ethyl formate-formic acid (20:20:0.1) | Chinese Pharmacopoeia (2020) |
| Wuweizi (Fructus Schisandrae Chinensis), Schisandrin | Methylbenzene-ethyl acetate (6:4) | ||
| Gouqizi (Fructus Lycii) | Ethyl acetate-trichloromethane-formic acid (3:2:1) | Xin Yao Zhuan Zheng Biao Zhun | |
| Tusizi (Semen Cuscutae) | Petroleum ether (30-60)-butyl acetate-butanone-methyl alcohol-water (15:25:3:7:1) | ||
| Schisandrin A | Methylbenzene-ethyl acetate (9:1) | ||
| Gallic acid | Methylbenzene-ethyl acetate-formic acid (6:3:1) | ||
| Wuzi Yanzong Pian | Tusizi (Semen Cuscutae) | Methylbenzene-ethyl acetate-formic acid (5:5:3) | Chinese Pharmacopoeia (2020) |
| Scopoletin | Petroleum ether (30-60)-ethyl formate-formic acid (20:20:0.1) | ||
| Wuweizi (Fructus Schisandrae Chinensis), Gomisin A | Methylbenzene-ethyl acetate (6:4) | ||
| Wuzi Yanzong Ruanjiaonang | Gouqizi (Fructus Lycii) | Petroleum ether (60-90)-ethyl acetate-formic acid (13:5:1) | Xin Yao Zhuan Zheng Biao Zhun |
| Tusizi (Semen Cuscutae) | Methylbenzene-ethyl acetate-formic acid (5:5:3) | ||
| Schisandrin A, Schisandrin | Petroleum ether (30-60)-ethyl acetate-formic acid (15:5:1) | Xin Yao Zhuan Zheng Biao Zhun | |
| Gouqizi (Fructus Lycii) | Acetate-trichloromethane-formic acid (2:3:0.1) | ||
| Quercetin | Acetate-trichloromethane-formic acid (26:13:5) | ||
| Wuzi Yanzong Jiaonang | Gouqizi (Fructus Lycii) | Trichloromethane-ethyl acetate-benzene-formic acid (5:6:3:1) | Xin Yao Zhuan Zheng Biao Zhun |
| Wuweizi (Fructus Schisandrae Chinensis) | Petroleum ethe (30-60)-ethyl acetate-formic acid (15:5:1) | ||
| Wuzi Yanzong Koufuye | Gouqizi (Fructus Lycii) | Trichloromethane-ethyl acetate-benzene-formic acid (5:6:3:1) | Zhong Yao Cheng Fang Zhi Ji |
Notes: TLC: thin layer chromatography.