Table 3.
HPLC methods developped for quantitative determination of Wuzi Yanzong prescription
| Dosage form | Determination of components | Detection method (wavelength) | Reference |
|---|---|---|---|
| Wuzi Yanzong Wan | Hyperoside, Gomisin A | HPLC (360, 250 nm) | Chinese Pharmacopoeia (2020) |
| Hyperoside, Kaempferide, Schisandrin, Schisandrin A, Schisandrin B | HPLC/PDA (360, 250 nm) | 23 | |
| Hyperoside, Quercitrin, kaempferol-3-rutinoside, Schisandrin, Schisandrin A, Schisandrin B | HPLC/PDA (254 nm) | 24 | |
| Betaine | HPLC (192 nm) | 25 | |
| Scopoletin | HPLC-FLD (343 nm,458 nm) | 26 | |
| Wuzi Yanzong Pian | Hyperoside, Gomisin A | HPLC (360，250 nm) | Chinese Pharmacopoeia (2020) |
| Schisandrin A | HPLC (230 nm) | Xin Yao Zhuan Zheng Biao Zhun | |
| Wuzi Yanzong Jiaonang | Schisandrin | HPLC (254 nm) | Xin Yao Zhuan Zheng Biao Zhun |
| Wuzi Yanzong Keli | Rutin | HPLC (254 nm) | Xin Yao Zhuan Zheng Biao Zhun |
Notes: HPLC: high performance liquid chromatography; PDA: photo-diode array.