Table 5.
Effects on the male reproductive system of Wuzi Yanzong prescription
| Pharmacological activity | Testing subject | Dosage | Effect | Reference |
|---|---|---|---|---|
| Effects on spermatogenesis | Oxidative damage of testis in mice | 2, 4 g·kg-1·d-1，35 d | Significantly antagonize the testicular oxidative injury and spermatogenic cell apoptosis induced by cyclophosphamide | 30 |
| Mice with oligospermia | 1.56 g·kg-1·d-1, 14 d | Regulate the meiosis of spermatogenic cells, homologous recombination of testis, renin-angiotensin recombination of testis, renin-angiotensin system, cholesterol metabolism pathway and others,so as to repair the blocking of testicular spermatogenesis,by affecting the gene expression of testis tissue in mice with oligospermia | 31 | |
| AT1KO mouse | 5.8 g/kg, 2 times a day | Has a positive regulatory effect on the sex hormone levels of male AT1+/- mice, increasing the levels of GnRH and FSH, and the degree of improvement is positively correlated with the intervention time, improving the reproductive function of male mice | 32 | |
| Testicular tissue of rats with kidney essence deficiency | 0.01 mL/g, 30 d | Can reduce the apoptosis of spermatogenic cells in rat testis by inhibiting Bax and promoting the expression of Bcl-2 protein | 33 | |
| Effects on sperm quality | Rat with oligospermia | 1.96 g·kg-1·d-1, 60 d | Can correct HPG secretion disorder and improve sperm quality | 34 |
| Effects on Sertoli cells | Mouse Testis TM4 Sertoli Cells | 12.0 g/kg, 2 times a day, 7 d | Serum can improve the secretory function of TM4 Sertoli cells by regulating the levels of ROS and autophagy | 35 |
| Human testicular support cell line TM4 cells | 0.2, 1.0, 5.0 mg/mL | Can improve oxidative stress injury of testicular support cells and inhibit cell apoptosis | 36 | |
| Heat stress model of rat Sertoli cells | 20 g/L | Can reduce the generation of reactive oxygen species and the expression of heat shock protein 70 to protect heat stress | 37 | |
| Effects on sperm mitochondria | Rats with Oligoasthenozoospermia | 1, 2, 4 g·kg-1·d-1, 28 d | Can inhibit the opening of mitochondrial permeability transition pore (MPTP), improve the quality of spermatozoa, and reduce the apoptosis rate of spermatogenic cells (including spermatozoa) | 38 |
| Rats with Oligoasthenozoospermia | 4.0g·kg-1·d-1, 28 d | Can down-regulate the expression of Bax,VDAC1 and CypD proteins in rat testicular tissue, inhibit the over-opening of mPTP, and prevent the germ cell apoptosis caused by the activation of Caspase protein family | 39 |
Notes: GnRH: gonadotropin-releasing hormone; HPG: human pituitary gonadotropin; TM4: testicular Sertolicells of normalmice; ROS: reactive oxygen species; MPTP: mitochondrial permeability transition pore; CypD: cyclophilin D; VDAC1: voltage-dependent anion-selective channel protein 1.