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. 2023 Feb 28;11:1106566. doi: 10.3389/fbioe.2023.1106566

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Copyright © 2023 Dianat, Münchberg, Blank, Freier and Ebert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Qualitative determination of the hydrophobic molecule content of engineered yeast. (A, B): Fluorescence intensity [-] at Ex/Em 515/583 nm (A), 550/638 nm (B) of Nile red-stained cell suspensions of the reference strain (Ref), and the two betulinic acid-producing strains BA6 and BA6∆ sampled from a batch cultivation during growth on glucose (I) and after the diauxic shift during growth on ethanol (II). The OD₆₀₀ of all samples was adjusted to 1. (C) Triterpenoid yield [mg/g_CDW] of the reference strain (Ref) and the two betulinic acid-producing strains BA6 and BA6∆ sampled from a batch cultivation during growth on glucose (I) and after the diauxic shift during growth on ethanol (II).