Table 2. Characteristics of 14 microsatellite markers developed for Pholiota microspora.
| Locus | Primer sequences (5'-3') | Repeat motif | Ta (°C) | Allele size range (bp) | Fluorescent label | Primer ratioa | Multiplex combination | A |
| Phmi01 | F-AAAGCTTATTTCACGCCAGG R-CGGCGTTAGTCGTTACATTG |
(ATC)10 | 59 | 85-94 | FAM | 1:7 | A | 4 |
| Phmi02 | F-TGTGCCCTTTCTCCTTGTCT R-TCCGAAACGCGATTTCTTAC** |
(AG)10 | 60 | 99-107 | VIC | 1:7 | A | 5 |
| Phmi03 | F-AGCTGGCGATCCCTGAAC R-GCCTCACCACCAGCCATC* |
(AGC)9 | 61 | 106-115 | PET | 1:3 | A | 4 |
| Phmi05 | F-GGGTTATCTCGATAATTTAACGGC R-GAGTCCATAAGTTTATCATCTAGCAAA |
(AG)9 | 61 | 102-110 | NED | 1:7 | A | 4 |
| Phmi07 | F-TACCTCAGCCACACTGGCA R-TGCCTTCAGTTCCGGAATAC** |
(AC)9 | 61 | 120-159 | FAM | 1:3 | B | 11 |
| Phmi08 | F-TTAACGGGTTTCTAGACCGC R-AAATCAATCTCAGAGCGCC |
(AC)9 | 59 | 135-147 | PET | 1:3 | B | 5 |
| Phmi09 | F-ACATGCGCATCATCGAAGT R-GCGCTGAGCTACAGTGAGACT* |
(AT)9 | 60 | 146-154 | VIC | 1:7 | B | 5 |
| Phmi10 | F-TAACACCACAGTTTGCATGG R-GACTTATGGTGCTGGCCC |
(AAC)9 | 58 | 133-145 | NED | 1:5 | B | 4 |
| Phmi13 | F-GGTTCCATGTCCGTGTTCTT R-GGGCTACCTTGCCTTCATCT |
(ACC)9 | 60 | 151-175 | PET | 1:3 | A | 8 |
| Phmi14 | F-GATGCCAGCCATTAGACGAT R-TGTATCCGCTTTCCTCTTGC** |
(AGC)10 | 60 | 142-183 | VIC | 1:7 | A | 8 |
| Phmi17 | F-CCGAGTGAACATATCCAGCA R-TTGTCTTCCTCCCTTCCTGA |
(AC)12 | 60 | 213-238 | FAM | 1:3 | A | 16 |
| Phmi20 | F-CGCTTAGAGGCACCAATACG R-GCTGAACGTGGACACTCTGA |
(AT)9 | 61 | 282-292 | FAM | 1:3 | B | 7 |
| Phmi23 | F-ACAAGACCCAACGTACAGGG R-ACAGGTGAAAGGGTCATGGA |
(AGG)9 | 60 | 286-313 | NED | 1:7 | B | 5 |
| Phmi24 | F-GCAATCTCATGAGCCTTTCA R-GAGATCCTGAAGGTTGTGACG |
(AT)9 | 59 | 309-321 | FAM | 1:3 | A | 7 |
Ta, annealing temperature; A, number of alleles across the 123 samples analyzed.
a Ratio of fluorescent and unlabeled forward primers for multiplex PCR. See text for details.
*, modified with a “PIG-tail” (GTTTCTT) at the 5' end (Brownstein et al., 1996). **, modified with an adjusted “PIG-tail” (GTTTCT) at the 5' end.