Table 1.

Primer pairs for quantitative RT-PCR

Primer	Direction	Sequence (5′→3′)	Position (mRNA)	Amplicon size (bp)
18S rRNA	For	GGA CAC GGA CAG GAT TGA CA	1278	50
18S rRNA	Rev	ACC CAC GGA ATC GAG AAA GA	1327
Insulin	For	TTC TAC ACA CCC AAG TCC CGT C	145	135
Insulin	Rev	ATC CAC AAT GCC ACG CTT CTG C	279
Insulin receptor	For	TGA CAA TGA GGA ATG TGG GGA C	875	129
Insulin receptor	Rev	GGG CAA ACT TTC TGA CAA TGA CTG	1003
IGF-I	For	GAC CAA GGG GCT TTT ACT TCA AC	65	127
IGF-I	Rev	TTT GTA GGC TTC AGC GGA GCA C	191
IGF-I receptor	For	GAA GTC TGC GGT GGT GAT AAA GG	2138	113
IGF-I receptor	Rev	TCT GGG CAC AAA GAT GGA GTT G	2250
IGF-II	For	CCA AGA AGA AAG GAA GGG GAC C	763	95
IGF-II	Rev	GGC GGC TAT TGT TGT TCA CAG C	857
IGF-II receptor	For	TTG CTA TTG ACC TTA GTC CCT TGG	1066	91
IGF-II receptor	Rev	AGA GTG AGA CCT TTG TGT CCC CAC	1156
IRS-1	For	GAT ACC GAT GGC TTC TCA GAC G	604	134
IRS-1	Rev	TCG TTC TCA TAA TAC TCC AGG CG	737
IRS-2	For	CAA CAT TGA CTT TGG TGA AGG GG	255	109
IRS-2	Rev	TGA AGC AGG ACT ACT GGC TGA GAG	363
IRS-4	For	ACC TGA AGA TAA GGG GTC GTC TGC	2409	132
IRS-4	Rev	TGT GTG GGG TTT AGT GGT CTG G	2540
AAH	For	TGC CTG CTC GTC TTG TTT GTG	1650	256
AAH	Rev	ATC CGT TCT GTA ACC CGT TGG	1906

AAH, aspartyl-(asparaginyl)-β-hydroxylase; IGF, insulin-like growth factor; IRS, insulin receptor substrate; RT-PCR, reverse transcriptase polymerase chain reaction.