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Primer sequence
| Gene | Primer sequence (5′-3′) |
|---|---|
| SIRT1 | Forward:TGGCAAAGGAGCAGATTAGTAGG |
| Reverse:CTGCCACAAGAACTAGAGGATAAGA | |
| microrna-34a | Forward:CGTCACCTCTTAGGCTTGGA |
| Reverse:CATTGGTGTCGTTGTGCT | |
| GAPDH | Forward:ATTCCACCCATGGCAAATTC |
| Reverse:TGGGATTTCCATTGATGACAAG | |
| U6 | Forward:CTCGCTTCGGCAGCACATATACT |
| Reverse:ACGCTTCACGAATTTGCGTGTC |
