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Dose–response curve for PLA2 hydrolysis of the lipid bilayer with 80:15:5 DMPC/DMPG/RPE lipo-beads at 30 °C. The enzyme activity at different concentrations of PLA2, ranging from 1 nM to 1 μM, was calculated as the percent decrease of the fluorescence intensity of lipo-beads using eq 1.
