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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immunoprecipitated PBMC lysates. Lane M, BenchMark prestained protein ladder (Life Technologies, Rockville, Md.); lane A, CACT114A (anti-ACT3)-immunoprecipitated proteins; lane B, ColiS169B (isotype control)-immunoprecipitated proteins. The arrow in lane A indicates the location of the protein submitted for N-terminal sequencing.
