Table 1.

Insulin secretion-related proteins identified in purified autophagosomes of MIN6 cells.

Cargos

INS (insulin) IAPP (islet amyloid polypeptide) CHGA (chromogranin A) CHGB/secretogranin-1 (chromogranin B) SCG2/CHGC(secretogranin II)) SCG3 (secretogranin III) SCG5 (secretogranin V) IGF2 (insulin-like growth factor 2) MIF (macrophage migration inhibitory factor (glycosylation-inhibiting factor))

Metabolic enzymes

PCSK1 (proprotein convertase subtilisin/kexin type 1) PCSK2 (proprotein convertase subtilisin/kexin type 2) CPE (carboxypeptidase E)

Transmembrane proteins

PTPRN (protein tyrosine phosphatase, receptor type, N) PTPRN2 (protein tyrosine phosphatase, receptor type, N polypeptide 2) SYT5 (synaptotagmin V) VAMP2 (vesicle-associated membrane protein 2) VAMP3 (vesicle-associated membrane protein 3) VAMP7 (vesicle-associated membrane protein 7) VAMP8 (vesicle-associated membrane protein 8)

RAB proteins

RAB3A (RAB3A, member RAS oncogene family) RAB27A (RAB27A, member RAS oncogene family) RAB37 (RAB37, member RAS oncogene family)

Ion channel proteins

CLCN3 (chloride channel, voltage-sensitive 3) SLC30A8/ZnT8 (solute carrier family 30 (zinc transporter), member 8) ATP6AP1 (ATPase, H+ transporting, lysosomal accessory protein 1)

The autophagosomes were purified from mouse MIN6 cells after HG treatment for 1 h.