Figure 2.

RAB21 KO cells are more sensitive to energy stress. (A-E) WT and RAB21 KO HeLa cells were cultured in FM or glucose-free (−Glc.) medium. (F-J) WT and RAB21 KO HeLa cells were cultured in FM or low glucose (1 mM) medium. (K-O) WT and RAB21 KO HeLa cells were cultured in serum-free (−FBS) medium. Representative images are shown. Cell death was quantified by flow cytometry through PI or ANXA5-FITC staining, and the results from three independent experiments were statistically analyzed. Scale bars: 100 μm. The graphs express the mean ± SEM. **P <0.01.