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. 2022 Sep 21;19(4):1277–1292. doi: 10.1080/15548627.2022.2124500

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Nfs are enriched in AP, and AL fractions, and infusion of 3-MA and MG-115 into mouse brain increases NF protein levels in vivo. (A) Organelle fractions (20 µg) from mouse brain except for total homogenate (Homog. 7 µg) were immunoblotted with NEFM, NEFH_COOH, NEFL, INA, MAP1LC3, CTSD and TOMM20 antibodies. Pon. S- Ponceau S stain. ICV infusion of 3-MA (9 mg/ml) into the right ventricle of mice and measurement of NEFM (B, 1.3-fold, n = 3, p < 0.0017), NEFH (B, NEFH_COOH, 2.1-fold, n = 3, p < 0.0114), NEFL (B, n = 3, p = 0.9066), MAP1LC3-I (B, n = 3, p = 0.0142), MAP1LC3-II (B, n = 3, p = 0.0090), the ratio of MAP1LC3-II:MAP1LC3-I (B, n = 3, p = 0.0967), ubiquitin (B, n = 3, p = 0.0874), the ratio of calpain-cleaved 150-kDa-SPTA1 fragment:FL-SPTA1 (B, n = 3, p = 0.0508) and CANX (B, n = 3, p = 0.5436) proteins. ICV Infusion of MG-115 (0.1 mg/ml) into the right ventricle and measurement of NEFM (C, 1.4-fold, n = 3, p < 0.0011), NEFL (C, n = 3, p = 0.1349), NEFH (C, NEFH_COOH, n = 3, p = 0.5212), Ubiquitin (C, n = 3, p < 0.0010), MAP1LC3-I (C, n = 3, p < 0.0145), MAP1LC3-II (C, n = 3, p < 0.0220), the ratio of MAP1LC3-II:MAP1LC3-I (C, n = 3, p < 0.0489), the ratio of calpain-cleaved 150-kDa-SPTA1 fragment:FL-SPTA1 (C, n = 3, p < 0.0349) and GAPDH (C, n = 3, p = 0.3512) proteins. Veh: Vehicle. Panels B and C, unpaired t-test; *p < 0.05; **p < 0.01.