TABLE 1.
Bacterial strains and plasmids utilized in this study
| Strain or plasmid | Genotype and phenotypea or relevant characteristic(s) | Reference(s) |
|---|---|---|
| R. capsulatus | ||
| SB1003 | cbbLS+cbbM+cbbP+ PHa+ PHg+ RubisCO+ PRK+ | 58 |
| SBI/II | cbbLS cbbM cbbP+ PHa− PHg+ RubisCO− PRK+ | 40 |
| RCP | cbbLS cbbM cbbP+ PHa+ PHg+ RubisCO− PRK+ | 37, 50 |
| SBP | cbbLS+cbbM+cbbP PHa+/− PHg+ RubisCO− PRK− | 40 |
| SBP-PHC | cbbLS+cbbM+cbbP PHa+ PHg+ RubisCO− PRK−; derepresses nitrogenase system | 37, 50 |
| Plasmids | ||
| pRK2013 | Helper plasmid for triparental conjugation | 14 |
| PXLB | Translational fusion; cbbI::lacZ; Tetr | 40 |
| PXFB | Translational fusion; cbbII::lacZ; Tetr | 40 |
| pALS53 | Translational fusion; dorC::lacZ; Tetr | A. Shaw and A. G. McEwan unpublished data |
| pHU316 | Translational fusion; nifH::lacZ; Tetr | 21 |
| pVKD1 | R. sphaeroides cbbI translational promoter fusion to lacZ; Tetr | 6 |
a
PHa, photoheterotrophic growth with ammonia as the nitrogen source; PHg, photoheterotrophic growth with glutamate as the nitrogen source; PHa−, lack of photoheterotrophic growth with ammonia as the nitrogen source and requirement of an alternative electron acceptor (DMSO); PHa+/−, gradual photoheterotrophic growth (barely grows; slow doubling time compared to wild type).